Coding
Part:BBa_M10086
Designed by: Jennifer Brophy Group: Berkeley BioE140L - S09 (2009-05-01)
{˂CPG_L2!}
Circularly permutated OmpG with a backbone opening in loop 2 of the native protein.
Circular permutation re-orders the protein so that both termini are exposed to the extra-cellular environment.
CPG_L2 is an outer membrane carrier protein that can be used to display other proteins on the outer membrane. "Display proteins" can be fused to the N-termini of {<CPG_L2!} and should be functionally displayed on the outer membrane of E.coli.
Streptavidin binding assay template
Growth curve assay template
Silver binding assay template
Autoaggregation assay template
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 253
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
n/a | {˂CPG_L2!} |